pRSET a sfMatryoshCaMP6s
(Plasmid #100020)

• Purpose: Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
• Depositing Lab: Wolf Frommer
• Publication: Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2.

Backbone

• Vector backbone: pRSET A
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 2973
• Total vector size (bp): 4926
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sfMatryoshCaMP6s
• Alt name: sfGCaMP6s-LSSmOrange
• Alt name: sfMCaMP6s
• Insert Size (bp): 1953
• Mutation: cpEGFP replaced with superfolder cpGFP
• Promoter: T7
• Tags / Fusion Proteins:
  • 6x HIS tag (N terminal on backbone)
  • Xpress_EK tag (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Supplemental Documents:
  • pRSET a - sfMatryoshCaMP6s.gb
• A portion of this plasmid was derived from a plasmid made by: The M13 peptide and CaM from GCaMP6s (derived from Addgene plasmid #40753 - Douglas Kim) were used in the construction of this plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry