pET15b-sfGO-Matryoshka-LE-LP
(Plasmid #100018)

• Purpose: Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
• Depositing Lab: Wolf Frommer
• Publication: Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2.

Backbone

• Vector backbone: pET-15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5678
• Total vector size (bp): 7118
• Modifications to backbone: Thrombin cleavage site deleted
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cpsfGFP-LSSmOrange
• Alt name: sfGO-Matryoshka
• Species: Synthetic
• Insert Size (bp): 1440
• Mutation: Leu and Glu N-term linker and Leu and Pro C-term linker
• Promoter: T7
• Tag / Fusion Protein:
  • 6x HIS tag (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Supplemental Documents:
  • pET-15b - sfGO-Matroshka_LE-LP.gb
• A portion of this plasmid was derived from a plasmid made by: Steven Boxer Lab - Stanford University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry