NsvBa
(Plasmid
#100015)
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PurposeElectrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100015 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTracer-CMV2, IRES GFP
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 6200
- Total vector size (bp) 7100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNsvBa
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Alt nameNsvBa, Non-selective voltage gated ion channel
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SpeciesBacillus alcalophilus
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Insert Size (bp)934
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GenBank IDJX399518.1
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NsvBa was a gift from David Clapham & Paul DeCaen (Addgene plasmid # 100015 ; http://n2t.net/addgene:100015 ; RRID:Addgene_100015) -
For your References section:
Ionic selectivity and thermal adaptations within the voltage-gated sodium channel family of alkaliphilic Bacillus. DeCaen PG, Takahashi Y, Krulwich TA, Ito M, Clapham DE. Elife. 2014 Nov 11;3. doi: 10.7554/eLife.04387. 10.7554/eLife.04387 PubMed 25385530