pOD2047-bwmControl
(Plasmid #100006)

• Purpose: MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).
• Depositing Lab: Karen Oegema
• Publication: Wang et al Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.

Backbone

• Vector backbone: pCFJ151
• Backbone manufacturer: Christian Frøkjær-Jensen (Erik Jorgensen lab)
• Backbone size w/o insert (bp): 7328
• Total vector size (bp): 14163
• Vector type: Targeting vector for Mos1 transposon mediated single copy transgene insertion
• Selectable markers: Cb-unc-119(+)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: zif-1
• Species: C. elegans (nematode)
• Insert Size (bp): 1594
• Entrez Gene: zif-1 (a.k.a. CELE_F59B2.6)

Gene/Insert 2

• Gene/Insert name: unc-119
• Species: C. briggsae
• Insert Size (bp): 1971

Resource Information

• Supplemental Documents:
  • pOD2047-bwmDEGcontrol.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry