pOD2045-intControl
(Plasmid
#100005)
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PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCFJ151
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Backbone manufacturerChristian Frøkjær-Jensen (Erik Jorgensen lab)
- Backbone size w/o insert (bp) 7328
- Total vector size (bp) 14163
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Vector typeTargeting vector for Mos1 transposon mediated single copy transgene insertion
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Selectable markersCb-unc-119(+)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namezif-1
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SpeciesC. elegans (nematode)
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Insert Size (bp)1594
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Entrez Genezif-1 (a.k.a. CELE_F59B2.6)
Gene/Insert 2
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Gene/Insert nameunc-119
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SpeciesC. briggsae
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Insert Size (bp)1971
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOD2045-intControl was a gift from Karen Oegema (Addgene plasmid # 100005 ; http://n2t.net/addgene:100005 ; RRID:Addgene_100005) -
For your References section:
A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B, Chisholm AD, Desai A, Oegema K. Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094. 10.1242/dev.150094 PubMed 28619826