His-NavMs
(Plasmid #100004)

• Purpose: Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
• Depositing Lab: Paul DeCaen
• Publication: Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10.1038/ncomms14205.

Backbone

• Vector backbone: pTracer-CMV2, IRES GFP
• Backbone manufacturer: Thermofisher
• Backbone size w/o insert (bp): 6200
• Total vector size (bp): 7100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: His-NavMs, Voltage gated sodium channel
• Species: Magnetococcus marinus
• Insert Size (bp): 882
• Mutation: C-terminus from a codon-optimized synthetic gene, starting at residue H237
• GenBank ID: CP000471.1
• Promoter: CMV
• Tag / Fusion Protein:
  • Hexa-His tag followed by a thrombin cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The GFP variant in this plasmid is missing the last five amino acids compared to the closest reference sequence (AAC53663.1). The depositor has confirmed that this does not impact plasmid function.