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Addgene

pACT2-Dhr1
(Plasmid #100003)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100003 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACT2
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dhr1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ECM16 (a.k.a. DHR1)
  • Tag / Fusion Protein
    • Y2H activation domain (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACT2-Dhr1 was a gift from Susan Baserga (Addgene plasmid # 100003 ; http://n2t.net/addgene:100003 ; RRID:Addgene_100003)

  • For your References section:

    The SSU processome interactome in Saccharomyces cerevisiae reveals potential new protein subcomplexes. Vincent NG, Charette JM, Baserga SJ. RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117. 10.1261/rna.062927.117 PubMed 29054886
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