The Expanded EasyClone-MarkerFree toolkit
(Kit #
1000000190
)
Depositing Lab: Irina Borodina
The Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites. Babaei M, Sartori L, Karpukhin A, Abashkin D, Matrosova E, Borodina I. FEMS Yeast Res. 2021 May 11;21(4):foab027. doi: 10.1093/femsyr/foab027. PubMed: PMID: 33893795 .
Description
The Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae. The markerless integration is enabled by CRISPR-Cas9 system. The workflow in the expanded toolkit is designed to be similar and compatible with the original EasyClone-MarkerFree Vector Set (Jessop-Fabre et al. 2016)
The Expanded EasyClone-MarkerFree toolkit. Figure from Babaei et al. FEMS Yeast Res. 2021 licensed under CC-BY license (Link opens in a new window).
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Expanded EasyClone-MarkerFree toolkit was a gift from Irina Borodina (Addgene kit #1000000190).”
For your Reference section:
Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites. Babaei M, Sartori L, Karpukhin A, Abashkin D, Matrosova E, Borodina I. FEMS Yeast Res. 2021 May 11;21(4):foab027. doi: 10.1093/femsyr/foab027. PubMed: PMID: 33893795 .
The Expanded EasyClone-MarkerFree toolkit - #1000000190
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pCfB9336 (pgRNA_II-1_NatMX) | Ampicillin |
| A / 2 | pCfB9337 (pgRNA_IV-1_NatMX) | Ampicillin |
| A / 3 | pCfB9339 (pgRNA_VII-1_NatMX) | Ampicillin |
| A / 4 | pCfB9340 (pgRNA_VIII-1_NatMX) | Ampicillin |
| A / 5 | pCfB9341 (pgRNA_IX-1_NatMX) | Ampicillin |
| A / 6 | pCfB9342 (pgRNA_XIII-1_NatMX) | Ampicillin |
| A / 7 | pCfB9343 (pgRNA_XV-1_NatMX) | Ampicillin |
| A / 8 | pCfB9344 (pgRNA_XVI-1_NatMX) | Ampicillin |
| A / 9 | pCfB9355(II-1_Markerfree_BackBone) | Ampicillin |
| A / 10 | pCfB9356(IV-1_Markerfree_BackBone) | Ampicillin |
| A / 11 | pCfB9358(VII-1_Markerfree_BackBone) | Ampicillin |
| A / 12 | pCfB9359(VIII-1_Markerfree_BackBone) | Ampicillin |
| B / 1 | pCfB9360(IX-1_Markerfree_BackBone) | Ampicillin |
| B / 2 | pCfB9361(XIII-1_Markerfree_BackBone) | Ampicillin |
| B / 3 | pCfB9362(XV-1_Markerfree_BackBone) | Ampicillin |
| B / 4 | pCfB9363(XVI-1_Markerfree_BackBone) | Ampicillin |