Skip to main content
Addgene

SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library
(Pooled Library #1000000172)

Print
  • Purpose

    This pair of duplicate libraries contains SARS-CoV-2 spike receptor binding domain (RBD) mutants in a yeast surface display vector.

    Each library is barcoded with randomized N16 barcodes downstream of the coding sequence.

    These libraries can be used for comprehensive mapping of the biochemical effects of RBD mutations.

  • Vector Backbone

    pETcon_SARS-CoV-2_RBD (Plasmid #166782)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000172 SARS-CoV-2_RBD_DMS_Library 1 $540 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Mutagenized CDS
    SARS-CoV-2 Receptor Binding Domain
  • Mutagenized region size
    603 bp
  • Total insert size
    1040 bp
  • Effective diversity
    ~150,000

Library Shipping

This library will be delivered as two pooled DNA libraries in microcentrifuge tubes on blue ice (tubes labeled lib1 and lib2). The tube contents will not necessarily be frozen. For best results, minimize freeze/thaws.

For each library tube, you will receive:

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

A scheme illustrating library assembly and use. In step one, a cartoon representation of PCR-based codon mutagenesis showing DNA with NNS sites leading to a library of mutant variants. In step three, random barcode sequence is appended to each mutant gene, and PacBio sequencing is used to generate long reads spanning barcode and mutant gene to generate a barcode-variant lookup table. The barcoded gene library is then expressed in a yeast display system, and Illumina sequencing of short barcodes can be used to read out the mutant variant in experimental partitions.

Figure 1: Bloom Lab SARS-CoV-2 RBD DMS Library design and application. Reproduced from Starr et al. 2020 under the Creative Commons Attribution 4.0 International License (CC BY 4.0) (Link opens in a new window).

The depositing laboratory provides the following GitHub repositories:

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library was a gift from Jesse Bloom (Addgene #1000000172)

  • For your References section:

    Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding. Starr TN, Greaney AJ, Hilton SK, Ellis D, Crawford KHD, Dingens AS, Navarro MJ, Bowen JE, Tortorici MA, Walls AC, King NP, Veesler D, Bloom JD Cell. 2020 Sep 3;182(5):1295-1310.e20. doi: 10.1016/j.cell.2020.08.012. Epub 2020 Aug 11. PubMed 32841599 (Link opens in a new window)
Related items
Additional resources