OVC Core and Extended Collections
(Kit #
1000000164, 1000000165
)
Depositing Lab: Harald Janovjak
The Janovjak Lab has provided both a Core and Extended Optogenetic Vector Collection (OVC).
Kit #1000000164
The Core Collection contains in total 24 plasmids: three plasmids containing the empty cloning cassette in configurations ABC, BAC or ACB, and 21 plasmids containing one of nine light-sensing domains (LSDs) or LSD binding partners in A as well as separately in site C (N- and C-terminally, respectively).
Kit #1000000165
The Extended Collection contains in total 17 plasmids: Four plasmids containing the cloning cassette with alternative linker configurations in site B, eight plasmids containing one of two LSDs engineered to heterodimerize and their binding partners, and five plasmids containing different constructs of a light-activated Opto-caspase 9.
These kits will be sent as bacterial glycerol stocks in 96-well plate format.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Kit | 1000000164 | OVC Core Collection (24 constructs) | 1 | $400 | Add to Cart | |
| Kit | 1000000165 | OVC Extended Collection (17 constructs) | 1 | $400 | Add to Cart | |
Original Publication
Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions Tichy A-M, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H. J Mol Biol.2019 Aug 9;431(17):3046-3055. doi: 10.1016/j.jmb.2019.05.033. PubMed PMID: 31150735.
Description
The Optogenetic Vector Collection (OVC) contains two sets of plasmids (the Core and the Extended Collection) to rapidly generate custom-made optogenetic tools for the manipulation of protein-protein interactions. The vectors are based on a modular cloning cassette (the primary cassette is termed ABC) containing three insertion sites (A, B, and C). Restriction sites A and C (AgeI and XmaI, respectively) generate compatible cohesive ends so that the target sequence can be inserted both N- or C-terminally using only one PCR product.
OVC Core Collection: (A) ABC and alternative cassettes. Cassettes include Kozak sequence, start codon and stop codon. (B) Cloning strategy based on the vector collection allows universal target amplicon insertion both N- and C-terminally. (C) LSDs and binding partners included in the OVC Core Collection.
OVC Extended Collection: (A) ABC cassettes with alternative linkers. (B) Heterodimerizing LSDs based on AsLOV2 and their binding partners (C) Opto-casp9 constructs with N- and C-terminal fusion of the LSD to the casp9 domain included in the OVC Extended Collection.
Kit Documentation
Workflow 192.9Â KBHow to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“OVC Core Collection was a gift from Harald Janovjak (Addgene kit #1000000164).”
“OVC Extended Collection was a gift from Harald Janovjak (Addgene kit #1000000165).”
For your Reference section:
Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions Tichy A-M, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H. J Mol Biol.2019 Aug 9;431(17):3046-3055. doi: 10.1016/j.jmb.2019.05.033. PubMed PMID: 31150735.
OVC Core Collection - #1000000164
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pOVC1 | Ampicillin |
| A / 2 | pOVC2 | Ampicillin |
| A / 3 | pOVC3 | Ampicillin |
| A / 4 | pOVC1_VfAU1-LOV_X | Ampicillin |
| A / 5 | pOVC1_CrPH-LOV_X | Ampicillin |
| A / 6 | pOVC1_NcVVD-LOV_X | Ampicillin |
| A / 7 | pOVC1_RsLP-LOV_X | Ampicillin |
| A / 8 | pOVC1_MxCarH-CBD_X | Ampicillin |
| A / 9 | pOVC1_TtCarH-CBD_X | Ampicillin |
| A / 10 | pOVC1_AtCRY2-PHR_X | Ampicillin |
| A / 11 | pOVC1_AtCIB_X | Ampicillin |
| A / 12 | pOVC1_AtPIF6_X | Ampicillin |
| B / 1 | pOVC1_AtPHYB-S_X | Ampicillin |
| B / 2 | pOVC1_ScPH1-S_X | Ampicillin |
| B / 3 | pOVC1_X_VfAU1-LOV | Ampicillin |
| B / 4 | pOVC1_X_CrPH-LOV | Ampicillin |
| B / 5 | pOVC1_X_NcVVD-LOV | Ampicillin |
| B / 6 | pOVC1_X_RsLP-LOV | Ampicillin |
| B / 7 | pOVC1_X_MxCarH-CBD | Ampicillin |
| B / 8 | pOVC1_X_TtCarH-CBD | Ampicillin |
| B / 9 | pOVC1_X_AtCRY2-PHR | Ampicillin |
| B / 10 | pOVC1_X_AtPIF6 | Ampicillin |
| B / 11 | pOVC1_X_ScPH1-S | Ampicillin |
| B / 12 | pOVC1_X_AtCIB | Ampicillin |
Kit Plate Map - #1000000164
OVC Extended Collection - #1000000165
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pOVC1_L1(GSSSG) | Ampicillin |
| A / 2 | pOVC1_L2(GSSGGSSG) | Ampicillin |
| A / 3 | pOVC1_L3(GSSSSGGSSSG) | Ampicillin |
| A / 4 | pOVC1_L4(EAAAK)3 | Ampicillin |
| A / 5 | pOVC1_EcSSPB-nano_X | Ampicillin |
| A / 6 | pOVC1_EcSSPB-micro_X | Ampicillin |
| A / 7 | pOVC1_HsPDZ1b_X | Ampicillin |
| A / 8 | pOVC1_X_AsPT1-LOV2-pep | Ampicillin |
| A / 9 | pOVC1_X_HsPDZ1b | Ampicillin |
| A / 10 | pOVC1_X_AsPT1-LOV2-EcSsra | Ampicillin |
| A / 11 | pOVC1_X_EcSSPB-micro | Ampicillin |
| A / 12 | pOVC1_X_EcSSPB-nano | Ampicillin |
| B / 1 | Opto-casp9_VfAU1-LOV_casp9 | Ampicillin |
| B / 2 | Opto-casp9_AtCRY2-PHR-casp9 | Ampicillin |
| B / 3 | Opto-casp9_casp9-VfAU1-LOV | Ampicillin |
| B / 4 | Opto-casp9_casp9-AtCRY2-PHR | Ampicillin |
| B / 5 | VfAU1-LOV_P2A_casp9 | Ampicillin |