Liu Human CRISPR Knockout Library
(Pooled library #1000000132)

• Purpose:

  This lentiviral genome-scale CRISPR library targets 18,436 human genes with 185,634 guide RNAs. The library is divided into 2 parts (h1 and h2). The h1 and h2 libraries contain in total 10 gRNAs per gene (5 gRNAs in each half library).

  gRNA design for this library is optimized to (1) maximize on-target cleavage effects using the SSC algorithm developed by the Liu Lab ( Xu et al. Genome Research 2015), (2) minimize potential off-target effects, and (3) ensure the fidelity of their sequence with corresponding target loci and avoid regions with possible genomic variations.

• Vector Backbone:

  lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

• Depositing Labs: Xiaole Shirley Liu
• Publication: Unpublished (How to cite )

Library Details

• Species: Human
• Genes targeted: 18,436
• gRNAs: 92,817 in each half library
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (DOCX, 145 KB)
  • Screen Protocol (DOCX, 165 KB)
• Depositor Data:
  • H1 gRNA sequences (XLSX, 3.5 MB)
  • H2 gRNA sequences (XLSX, 3.5 MB)
  • H1 gRNA Distribution Raw Count (TXT, 1.8 MB)
  • H2 gRNA Distribution Raw Count (TXT, 1.8 MB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.

Addgene recommends using electrocompetent cells for amplification to ensure 50X coverage.