pCri System
(Kit #
1000000058
)
Depositing Lab: F. Xavier Gomis-Rüth
pCri System plasmids can be utilized for the cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
The pCri System: a vector collection for recombinant protein expression and purification. Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, Arolas JL, Gomis-Rüth FX. PLoS One 2014 Nov 11;9(11):e112643. doi: 10.1371/journal.pone.0112643. eCollection 2014. PubMed .
Description
pCri System plasmids can be utilized for the cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli , Bacillus subtilis , and Pichia pastoris . These constructs are designed for directional cloning and allow for the incorporation of different fusion tags, signal peptides, and cleavage sites at the N- and C-terminus of proteins.
This versatile system includes a range of fusion tags suitable for different affinity purification methods, and the simple cloning approach makes this a very useful system for high throughput studies.
Kit Documentation
Documents included with shipment: pCri System plasmid kit info 129.1 KB
Additional Reference
The LSL tag found in pCri14 was kindly provided by Dr. José Miguel Mancheño. Please, cite the following reference when using this tag:
High-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile beta-trefoil lectin domain from the mushroom Laetiporus sulphurous. Angulo I, Acebrón I, de las Rivas B, Muñoz R, Rodríguez-Crespo I, Menéndez M, García P, Tateno H, Goldstein IJ, Pérez-Agote B, Mancheño JM. Glycobiology 2011 21: 1349–1361 PubMed .
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The pCri System plasmid kit was a gift from F. Xavier Gomis-Rüth (Addgene kit # 1000000058).”
For your Reference section:
The pCri System: a vector collection for recombinant protein expression and purification. Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, Arolas JL, Gomis-Rüth FX. PLoS One 2014 Nov 11;9(11):e112643. doi: 10.1371/journal.pone.0112643. eCollection 2014. PubMed .
pCri System - #1000000058
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Bleocin (Zeocin) | |
| Kanamycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pCri-12b | Ampicillin |
| A / 2 | pCri-12a | Ampicillin |
| A / 3 | pCri-17a | Ampicillin |
| A / 4 | pCri-18a | Ampicillin |
| A / 5 | pCri-15a | Bleocin (Zeocin) |
| A / 6 | pCri-16a | Bleocin (Zeocin) |
| A / 7 | pCri-15b | Bleocin (Zeocin) |
| A / 8 | pCri-1a | Kanamycin |
| A / 9 | pCri-4a | Kanamycin |
| A / 10 | pCri-6a | Kanamycin |
| A / 11 | pCri-7a | Kanamycin |
| A / 12 | pCri-8a | Kanamycin |
| B / 1 | pCri-9a | Kanamycin |
| B / 2 | pCri-11a | Kanamycin |
| B / 3 | pCri-13a | Kanamycin |
| B / 4 | pCri-14a | Kanamycin |
| B / 5 | pCri-1b | Kanamycin |
| B / 6 | pCri-4b | Kanamycin |
| B / 7 | pCri-6b | Kanamycin |
| B / 8 | pCri-7b | Kanamycin |
| B / 9 | pCri-8b | Kanamycin |
| B / 10 | pCri-9b | Kanamycin |
| B / 11 | pCri-11b | Kanamycin |
| B / 12 | pCri-14b | Kanamycin |
| C / 1 | pCri-1a-Strep | Kanamycin |
| C / 2 | pCri-4a-Strep | Kanamycin |
| C / 3 | pCri-7a-Strep | Kanamycin |
| C / 4 | pCri-8a-Strep | Kanamycin |
| C / 5 | pCri-9a-Strep | Kanamycin |