MoClo Plant Parts Kit
(Kit #
1000000047
)
Depositing Labs:
Nicola Patron
This modular cloning (MoClo) collection comprises 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation. Thirty nine parts encode promoters and 5' untranslated regions; eleven parts encode antigenic tags; eight parts encode sub-cellular localisation signals; nineteen parts encode reporter genes; six parts encode selectable marker genes; nine parts encode terminators and 3' untranslated regions. A suppressor of silencing and two linkers are also included.
This kit consists of 1 plate, and will be shipped to most countries as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
Original Publication
A Golden Gate Modular Cloning Toolbox for Plants. Engler C, Youles M, Gruetzner R, Ehnert T-M, Werner S, Jones JDG, Patron NJ, and Marillonnet S. ACS Synthetic Biology . 2014 Feb 20. doi: 10.1021/sb4001504. PubMed PMID 24933124.Description
This collection comprises 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation. Thirty nine parts encode promoters and 5' untranslated regions; eleven parts encode antigenic tags; eight parts encode sub-cellular localisation signals; nineteen parts encode reporter genes; six parts encode selectable marker genes; nine parts encode terminators and 3' untranslated regions. A suppressor of silencing and two linkers are also included.
Image from Weber et al., PLoS One. 2011 Feb 18;6(2):e16765.
Kit Documentation
Documents included with shipment:
MoClo Plant Parts Kit Info
1.2Â MB
Download a ZIP file containing all MoClo Plant Parts Kit plasmid sequences in GenBank format:
MoClo Plant Parts Kit GenBank Files
247.7Â KB
Protocols
See the original ACS Synthetic Biology publication (PubMed PMID 24933124) for detailed protocols for this kit.
Links
For additional information browse the SYNBIO@TSL resources that have been developed by Nicola Patron and colleagues at The Sainsbury Laboratory:
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The MoClo Plant Parts Kit was a gift from Nicola Patron (Addgene kit # 1000000047)"
For your Reference section:
A Golden Gate Modular Cloning Toolbox for Plants. Engler C, Youles M, Gruetzner R, Ehnert T-M, Werner S, Jones JDG, Patron NJ, and Marillonnet S. ACS Synthetic Biology. 2014 Feb 20. doi: 10.1021/sb4001504. PubMed PMID 24933124.
MoClo Plant Parts Kit - #1000000047
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Spectinomycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pICH41373 | Spectinomycin |
| A / 2 | pICH45180 | Spectinomycin |
| A / 3 | pICH51277 | Spectinomycin |
| A / 4 | pICH45234 | Spectinomycin |
| A / 5 | pICH87611 | Spectinomycin |
| A / 6 | pICH78133 | Spectinomycin |
| A / 7 | pICSL30008 | Spectinomycin |
| A / 8 | pICSL50007 | Spectinomycin |
| A / 9 | pICSL50004 | Spectinomycin |
| A / 10 | pICH49477 | Spectinomycin |
| A / 11 | pICSL80016 | Spectinomycin |
| A / 12 | pICH44300 | Spectinomycin |
| B / 1 | pICH41388 | Spectinomycin |
| B / 2 | pICH45125 | Spectinomycin |
| B / 3 | pICH51288 | Spectinomycin |
| B / 4 | pICH45244 | Spectinomycin |
| B / 5 | pICH41402 | Spectinomycin |
| B / 6 | pICH78141 | Spectinomycin |
| B / 7 | pICSL30009 | Spectinomycin |
| B / 9 | pICSL50011 | Spectinomycin |
| B / 10 | pICSL80004 | Spectinomycin |
| B / 11 | pICSL70002 | Spectinomycin |
| B / 12 | pICH77901 | Spectinomycin |
| C / 1 | pICH45089 | Spectinomycin |
| C / 2 | pICH45131 | Spectinomycin |
| C / 3 | pICSL12006 | Spectinomycin |
| C / 4 | pICH45214 | Spectinomycin |
| C / 5 | pICH44199 | Spectinomycin |
| C / 6 | pAGM5331 | Spectinomycin |
| C / 7 | pICSL30004 | Spectinomycin |
| C / 8 | pICSL50010 | Spectinomycin |
| C / 10 | pICSL80001 | Spectinomycin |
| C / 11 | pICSL70004 | Spectinomycin |
| C / 12 | pICH77911 | Spectinomycin |
| D / 1 | pICH42211 | Spectinomycin |
| D / 2 | pICH45152 | Spectinomycin |
| D / 3 | pICH87633 | Spectinomycin |
| D / 4 | pICH87655 | Spectinomycin |
| D / 5 | pICH44222 | Spectinomycin |
| D / 6 | pAGM1482 | Spectinomycin |
| D / 7 | pICSL30006 | Spectinomycin |
| D / 8 | pICSL50012 | Spectinomycin |
| D / 9 | pICSL50006 | Spectinomycin |
| D / 10 | pICH75111 | Spectinomycin |
| D / 11 | pICSL70005 | Spectinomycin |
| D / 12 | pICH41432 | Spectinomycin |
| E / 1 | pICH50581 | Spectinomycin |
| E / 2 | pICH41551 | Spectinomycin |
| E / 3 | pICH85281 | Spectinomycin |
| E / 4 | pICH71292 | Spectinomycin |
| E / 5 | pICH44233 | Spectinomycin |
| E / 6 | pAGM5355 | Spectinomycin |
| E / 7 | pICSL30003 | Spectinomycin |
| E / 8 | pICSL50013 | Spectinomycin |
| E / 9 | pICSL80014 | Spectinomycin |
| E / 10 | pICH42222 | Spectinomycin |
| E / 11 | pICSL70008 | Spectinomycin |
| E / 12 | pICH71431 | Spectinomycin |
| F / 1 | pICH42760 | Spectinomycin |
| F / 2 | pICSL13001 | Spectinomycin |
| F / 3 | pICH88103 | Spectinomycin |
| F / 4 | pICH71301 | Spectinomycin |
| F / 5 | pICH44188 | Spectinomycin |
| F / 6 | pAGM5343 | Spectinomycin |
| F / 7 | pICSL30010 | Spectinomycin |
| F / 8 | pICSL50014 | Spectinomycin |
| F / 9 | pICH41531 | Spectinomycin |
| F / 10 | pICH43844 | Spectinomycin |
| F / 11 | pICH41414 | Spectinomycin |
| F / 12 | pICH71411 | Spectinomycin |
| G / 1 | pICH44157 | Spectinomycin |
| G / 2 | pICSL13002 | Spectinomycin |
| G / 3 | pICH87644 | Spectinomycin |
| G / 4 | pICH71311 | Spectinomycin |
| G / 5 | pICH44179 | Spectinomycin |
| G / 6 | pAGM1467 | Spectinomycin |
| G / 7 | pICH37431 | Spectinomycin |
| G / 8 | pICSL50008 | Spectinomycin |
| G / 9 | pICSL80005 | Spectinomycin |
| G / 10 | pICH44022 | Spectinomycin |
| G / 11 | pICH72400 | Spectinomycin |
| G / 12 | pICH71421 | Spectinomycin |
| H / 1 | pICH45173 | Spectinomycin |
| H / 2 | pICH51266 | Spectinomycin |
| H / 3 | pICH45195 | Spectinomycin |
| H / 4 | pICH71342 | Spectinomycin |
| H / 5 | pAGM1479 | Spectinomycin |
| H / 6 | pICSL30005 | Spectinomycin |
| H / 7 | pICH37326 | Spectinomycin |
| H / 8 | pICSL50016 | Spectinomycin |
| H / 9 | pICSL80007 | Spectinomycin |
| H / 10 | pICSL80012 | Spectinomycin |
| H / 11 | pICH41421 | Spectinomycin |
| H / 12 | pAGT707 | Spectinomycin |