MoClo Toolkit
(Kit #
1000000044
)
Depositing Labs:
Sylvestre Marillonnet
This collection contains a set of 95 empty standardized genetic modules that can be used for hierarchical assembly based on the Golden Gate cloning technique. This kit was deposited by Sylvestre Marillonnet's lab and is comprised of plasmids for cloning: promoters, 5' untranslated regions, signal peptides, coding sequences, and terminators. The MoClo kit can be used to assemble any eukaryotic multigene construct in three steps.
This kit consists of 1 plate, and will be shipped to most countries as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
Original Publications
A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLOS ONE. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed PMID 21364738.Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system. Werner S, Engler C, Weber E, Gruetzner R, Marillonnet S. Bioeng Bugs. 2012 Jan 1;3(1):38-43. doi: 10.4161/bbug.3.1.18223. PubMed PMID 22126803.
Description
This collection contains a set of 95 empty standardized genetic modules that can be used for hierarchical assembly based on the Golden Gate cloning technique. This kit was deposited by Sylvestre Marillonnet's lab and is comprised of plasmids for cloning: promoters, 5' untranslated regions, signal peptides, coding sequences, and terminators. The MoClo kit can be used to assemble any eukaryotic multigene construct in three steps.
Image from Weber et al., PLoS One. 2011 Feb 18;6(2):e16765.
Kit Documentation
Documents included with shipment: MoClo Toolkit Plasmid Kit Info 1.2Â MB
Download a ZIP file containing all MoClo Kit plasmid sequences in GenBank format: MoClo Toolkit GenBank files 825.5Â KB
Download a PDF file containing figures illustrating the MoClo Kit constructs: Additional Figures 212.0Â KB
Protocols
See the original PLOS ONE publication (PubMed PMID 21364738) for detailed protocols for this kit.
Links
For additional information browse the SYNBIO@TSL resources that have been developed by Nicola Patron and colleagues at The Sainsbury Laboratory:
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The MoClo Toolkit was a gift from Sylvestre Marillonnet (Addgene kit # 1000000044)"
For your Reference section:
A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLOS ONE . 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed PMID 21364738.
Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system. Werner S, Engler C, Weber E, Gruetzner R, Marillonnet S. Bioeng Bugs. 2012 Jan 1;3(1):38-43. doi: 10.1371/journal.pone.0016765. PubMed PMID 22126803.
MoClo Tool Kit - #1000000044
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Kanamycin | |
| Spectinomycin | |
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pAGM1311 | Kanamycin |
| A / 2 | pICH53399 | Spectinomycin |
| A / 3 | pICH41331 | Spectinomycin |
| A / 4 | pICH47802 | Ampicillin |
| A / 5 | pAGM4723 | Kanamycin |
| A / 6 | pICH49255 | Ampicillin |
| A / 7 | pICH83977 | Ampicillin |
| A / 8 | pAGM8055 | Spectinomycin |
| A / 9 | pICH50900 | Ampicillin |
| A / 10 | pICH75366 | Kanamycin |
| A / 11 | pICH79300 | Ampicillin |
| A / 12 | pICH54077 | Ampicillin |
| B / 1 | pICH41233 | Spectinomycin |
| B / 2 | pICH41246 | Spectinomycin |
| B / 3 | pICH47732 | Ampicillin |
| B / 4 | pICH47811 | Ampicillin |
| B / 5 | pICH41722 | Spectinomycin |
| B / 6 | pICH49266 | Ampicillin |
| B / 7 | pICH83988 | Ampicillin |
| B / 8 | pAGM8067 | Spectinomycin |
| B / 9 | pICH50914 | Ampicillin |
| B / 10 | pICH75377 | Kanamycin |
| B / 11 | pICH79311 | Ampicillin |
| B / 12 | pICH89921 | Kanamycin |
| C / 1 | pAGM1263 | Spectinomycin |
| C / 2 | pICH41264 | Spectinomycin |
| C / 3 | pICH47742 | Ampicillin |
| C / 4 | pICH47822 | Ampicillin |
| C / 5 | pICH41744 | Spectinomycin |
| C / 6 | pICH49277 | Ampicillin |
| C / 7 | pICH83999 | Ampicillin |
| C / 8 | pAGM8079 | Spectinomycin |
| C / 9 | pICH50927 | Ampicillin |
| C / 10 | pICH75388 | Kanamycin |
| C / 11 | pICH54011 | Ampicillin |
| C / 12 | pICH82113 | Kanamycin |
| D / 1 | pAGM1276 | Spectinomycin |
| D / 2 | pICH41276 | Spectinomycin |
| D / 3 | pICH47751 | Ampicillin |
| D / 4 | pICH47831 | Ampicillin |
| D / 5 | pICH41766 | Spectinomycin |
| D / 6 | pICH49283 | Ampicillin |
| D / 7 | pICH84000 | Ampicillin |
| D / 8 | pAGM8081 | Spectinomycin |
| D / 9 | pICH50932 | Ampicillin |
| D / 10 | pICH79255 | Ampicillin |
| D / 11 | pICH54022 | Ampicillin |
| D / 12 | pICH82094 | Kanamycin |
| E / 1 | pICH41258 | Spectinomycin |
| E / 2 | pAGM1251 | Spectinomycin |
| E / 3 | pICH47761 | Ampicillin |
| E / 4 | pICH47841 | Ampicillin |
| E / 5 | pICH41780 | Spectinomycin |
| E / 6 | pICH49299 | Ampicillin |
| E / 7 | pICH84011 | Ampicillin |
| E / 8 | pAGM8093 | Spectinomycin |
| E / 9 | pICH75322 | Kanamycin |
| E / 10 | pICH79264 | Ampicillin |
| E / 11 | pICH54033 | Ampicillin |
| E / 12 | pICH86966 | Kanamycin |
| F / 1 | pAGM1299 | Spectinomycin |
| F / 2 | pAGM1287 | Spectinomycin |
| F / 3 | pICH47772 | Ampicillin |
| F / 4 | pICH47852 | Ampicillin |
| F / 5 | pICH41800 | Spectinomycin |
| F / 6 | pICH49300 | Ampicillin |
| F / 7 | pICH83955 | Ampicillin |
| F / 8 | pICH50872 | Ampicillin |
| F / 9 | pICH75334 | Kanamycin |
| F / 10 | pICH79277 | Ampicillin |
| F / 11 | pICH54044 | Ampicillin |
| F / 12 | pICH86988 | Kanamycin |
| G / 1 | pAGM1301 | Spectinomycin |
| G / 2 | pICH41295 | Spectinomycin |
| G / 3 | pICH47781 | Ampicillin |
| G / 4 | pICH47861 | Ampicillin |
| G / 5 | pICH41822 | Spectinomycin |
| G / 6 | pICH49244 | Ampicillin |
| G / 7 | pAGM8031 | Spectinomycin |
| G / 8 | pICH50881 | Ampicillin |
| G / 9 | pICH75344 | Kanamycin |
| G / 10 | pICH79289 | Ampicillin |
| G / 11 | pICH54055 | Ampicillin |
| G / 12 | pAGM9121 | Spectinomycin |
| H / 1 | pICH53388 | Spectinomycin |
| H / 2 | pICH41308 | Spectinomycin |
| H / 3 | pICH47791 | Ampicillin |
| H / 4 | pAGM4673 | Kanamycin |
| H / 5 | pICH50866 | Spectinomycin |
| H / 6 | pICH83966 | Ampicillin |
| H / 7 | pAGM8043 | Spectinomycin |
| H / 8 | pICH50892 | Ampicillin |
| H / 9 | pICH75355 | Kanamycin |
| H / 10 | pICH79290 | Ampicillin |
| H / 11 | pICH54066 | Ampicillin |