pDONR221-Sgd1
(Plasmid
#100000)
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PurposeShuttle Sgd1 into Gateway destination vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100000 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSgd1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneSGD1
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-Sgd1 was a gift from Susan Baserga (Addgene plasmid # 100000 ; http://n2t.net/addgene:100000 ; RRID:Addgene_100000) -
For your References section:
The SSU processome interactome in Saccharomyces cerevisiae reveals potential new protein subcomplexes. Vincent NG, Charette JM, Baserga SJ. RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117. 10.1261/rna.062927.117 PubMed 29054886