Showing: 61 - 80 of 124 results
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Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pNM58
Plasmid#128409PurposeSystem with AIP and xylose inducible agrCA sensorDepositorInsertsargCA
gfpmut2
UseTagsExpressionBacterialMutationPromoterP3 and PxylRAvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-SaODH
Plasmid#110804PurposeExpresses SaODH and opine dehydrogenase from Staphylococcus aureusDepositorInsertSAV2468 (SAV2468 )
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
GST-MNase
Plasmid#136291PurposeExpresses Micrococcal nucleaseDepositorInsertMNase
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSR1048
Plasmid#208738PurposeCharacterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)DepositorInsertPRelA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPRelAAvailabilityAcademic Institutions and Nonprofits only -
pSR1051
Plasmid#208739PurposeCharacterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)DepositorInsertPZwf
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPZwfAvailabilityAcademic Institutions and Nonprofits only -
pSR1054
Plasmid#208740PurposeCharacterization plasmid for PAmy (sfgfp expressed from PAmy on the S. aureus pC194 replicon)DepositorInsertPAmy
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPAmyAvailabilityAcademic Institutions and Nonprofits only -
pSR1055
Plasmid#208741PurposeCharacterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)DepositorInsertPHrcA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPHrcAAvailabilityAcademic Institutions and Nonprofits only -
pSR1057
Plasmid#208742PurposeCharacterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)DepositorInsertPPgk
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPPgkAvailabilityAcademic Institutions and Nonprofits only -
pSR1058
Plasmid#208743PurposeCharacterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)DepositorInsertPHbs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPHbsAvailabilityAcademic Institutions and Nonprofits only -
pRF-IcaR
Plasmid#49362DepositorInsertIcaR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRF-QacR
Plasmid#49369DepositorInsertQacR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET21a-IcaR-e
Plasmid#51176DepositorInsertIcaR
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
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pNM262
Plasmid#128412PurposeVanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseSynthetic BiologyTagsamyE SPExpressionBacterialMutationPromoterPspank(V)AvailabilityAcademic Institutions and Nonprofits only -
pSK9065
Plasmid#84345Purposetightly controlled expression of mRFP fusion proteins in S. aureusDepositorTypeEmpty backboneUseTagsmRFPmarsExpressionBacterialMutationPromoterPxyl/tetAvailabilityAcademic Institutions and Nonprofits only -
SAPDF-pET21a(+)
Plasmid#12649DepositorInsertSA Peptide deformylase
UseTagsHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGBHPS-SortA
Plasmid#21930DepositorInsertsortase
UseTagsGB1, HRV3C cleagage site, and HisExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 124 results