Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.
Xie K, Minkenberg B, Yang Y
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed Article
Xie K, Minkenberg B, Yang Y
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
63142 | pRGEB32 | Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation. |
63143 | pGTR | PCR template to construct polycistronic tRNA-gRNA |
63159 | pRGE32 | Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast |