pCMV-ARMS1-ProLink2-MRGPRX3
(Plasmid #213962)

• Purpose: Expresses MRGPRX3 in mammalian cells
• Depositing Lab: Christa Müller
• Publication: Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10.1038/s42003-023-05739-5.

Backbone

• Vector backbone: pCMV-ARMS1-ProLinkTM 2
• Backbone manufacturer: Eurofins DiscoverX, Fremont, CA, USA
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418), Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MRGPRX3
• Entrez Gene: MRGPRX3 (a.k.a. GPCR, MRGX3, SNSR1, SNSR2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: GCATAATGCTAGCACCATGGATTCAACCATCCCAGTCTTG
• 3′ sequencing primer: TTCGAAGCTTGACTGCTCCAATCTGCTTCCCGAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Christa Müller research group

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry