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Addgene

pSD5
(Plasmid #197289)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197289 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCC1BAC
  • Backbone size w/o insert (bp) 17996
  • Total vector size (bp) 19319
  • Modifications to backbone
    Addition of CEN-ARS-HIS sequences for replication and selection in S. cerevisiae
  • Vector type
    Synthetic Biology ; Bacterial and Yeast Cloning
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli Epi300
  • Growth instructions
    Plasmid can be induced to high copy with arabinose.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NptII
  • Alt name
    Neomycin resistance gene
  • Insert Size (bp)
    1323

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSD5 was a gift from Bogumil Karas (Addgene plasmid # 197289 ; http://n2t.net/addgene:197289 ; RRID:Addgene_197289)
  • For your References section:

    SLICER: A Seamless Gene Deletion Method for Deinococcus radiodurans. Brumwell SL, Van Belois KD, Nucifora DP, Karas BJ. Biodes Res. 2023 Mar 15;5:0009. doi: 10.34133/bdr.0009. eCollection 2023. 10.34133/bdr.0009 PubMed 37849465