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Addgene

pSK-c-mpl
(Plasmid #1828)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-mpl
  • Alt name
    murine myeloproliferative leukemia
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2935
  • Entrez Gene
    Mpl (a.k.a. CD110, TPO-R, c-mpl, hlb219)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See scanned map.
(Leder #C-320)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSK-c-mpl was a gift from Philip Leder (Addgene plasmid # 1828 ; http://n2t.net/addgene:1828 ; RRID:Addgene_1828)

  • For your References section:

    Murine c-mpl: a member of the hematopoietic growth factor receptor superfamily that transduces a proliferative signal. Skoda RC, Seldin DC, Chiang MK, Peichel CL, Vogt TF, Leder P. EMBO J 1993 Jul;12(7):2645-53. PubMed 8334987
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