Showing: 1 - 20 of 26666 results
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Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a MISTIC
Plasmid#85999Purposebacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tagDepositorInsertN/A
UseTagsHIS6 and MISTICExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 162-267
Plasmid#86002Purposebacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53DepositorTypeEmpty backboneUseTagsSUMOExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LVXN-Neo-NSD2
Plasmid#86010Purposeexpress wild-type NSD2 in mammalian cellsDepositorInsertNSD2 (NSD2 Human)
UseLentiviralTagsFlag tag D Y K D D D D KExpressionBacterialMutationK1002R (please see depositor comments below)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pNIC28-DENV3-NS5FL
Plasmid#86013PurposeExpression of dengue virus 3 NS5 proteinDepositorInsertDENV3-NS5FL
UseTagshexa-histidine tagExpressionBacterialMutationamino acid residues 6 to 895 of DENV3 NS5PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pQhsCDtRNA+
Plasmid#86014PurposeMulti-gene expression system for purification of human box C/D RNPs with U14-tRNA minigeneDepositorInsertsUseCo-expression; purificationTags6x-HisExpressionBacterialMutationamino acids 1-401, amino acids 1-411, and amino a…PromoterLPP and PtacAvailabilityAcademic Institutions and Nonprofits only -
pQhsCDtRNA-
Plasmid#86015PurposeMulti-gene expression system with human box C/D proteins with U14DepositorInsertsUseCo-expression; purificationTags6x-HisExpressionBacterialMutationamino acids 1-401, amino acids 1-411, and amino a…PromoterAvailabilityAcademic Institutions and Nonprofits only -
GST-thr-RAF1-RBD
Plasmid#86033PurposeExpresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activationDepositorInsertRAF1 RBD (Ras-binding domain) (RAF1 Human)
UseTagsGSTExpressionBacterialMutationContains residues 1-149PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
CFFΔC-Myc
Plasmid#86060PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.DepositorInsertFibrocystin (Pkhd1 Mouse)
UseTagsMycExpressionBacterialMutationcontains CD8a luminal and transmembrane domains a…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GST-cystin-CTS
Plasmid#86066PurposeA bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.DepositorInsertCystin (Cys1 Mouse)
UseTagsGSTExpressionBacterialMutationcontains the region from 28-35PromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-PC-1-CTS
Plasmid#86067PurposeA bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsGSTExpressionBacterialMutationcontains the region from 4297-4303PromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-PC-2-CTS
Plasmid#86068PurposeA bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-2 (Pkd2 Human)
UseTagsGSTExpressionBacterialMutationcontains the region from 1-15PromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-prRDH-CTS
Plasmid#86069PurposeA bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.DepositorInsertRetinol dehydrogenase (RDH8 Bovine)
UseTagsGSTExpressionBacterialMutationthe insert is 297-312 of RDH8 (Bos taurus)PromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-peripherin-CTS
Plasmid#86070PurposeA bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.DepositorInsertPeripherin (prph2.S Frog)
UseTagsGSTExpressionBacterialMutationcontains the region from 317-336 of peripherinPromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-rhodopsin-CTS
Plasmid#86071PurposeA bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.DepositorInsertRhodopsin (RHO Bovine)
UseTagsGSTExpressionBacterialMutationcontains the region from 309-348 of RhodopsinPromotertacAvailabilityAcademic Institutions and Nonprofits only -
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GST-RP2-C86Y,P95L
Plasmid#86074PurposeA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.DepositorInsertRP2 (RP2 Human)
UseTagsGSTExpressionBacterialMutationChanged cysteine-86 and proline-95 to tyrosine an…PromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-wt
Plasmid#86078PurposeA bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-QL
Plasmid#86079PurposeA bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationChanged glutamine 67 to leucinePromotertacAvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-TN
Plasmid#86080PurposeA bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationChanged threonine 22 to asparginePromotertacAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 26666 results