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Addgene
Showing: 1 - 20 of 26666 results
  1. pET28a SUMO Nup53xl 162-267

    Plasmid
    #86002
    Purpose
    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53
    Depositor
    Type
    Empty backbone
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Availability
    Academic Institutions and Nonprofits only
  3. GST-thr-RAF1-RBD

    Plasmid
    #86033
    Purpose
    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation
    Depositor
    Insert
    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Contains residues 1-149
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. CFFΔC-Myc

    Plasmid
    #86060
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.
    Depositor
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Use
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    contains CD8a luminal and transmembrane domains a…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. GST-prRDH-CTS

    Plasmid
    #86069
    Purpose
    A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Insert
    Retinol dehydrogenase (RDH8 Bovine)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    the insert is 297-312 of RDH8 (Bos taurus)
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 26666 results