Showing: 61 - 80 of 112 results
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Plasmid#25288PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert55.m00007:I39-E531
UseTagsHisExpressionBacterialMutationContains amino acids I39-E531PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pGCaMP5-DHFR
Plasmid#78706PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.DepositorInsertsGCaMP5
DHFR
UseTagsExpressionMutationPromoterToxoplasma SAG1 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
PP-CDPK1 (3DXN)
Plasmid#25129PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert541.m00134:MAC02T-C05:C40091
UseTagsHisExpressionBacterialMutationContains amino acids L72-Q339PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT
Plasmid#87259PurposeYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133DepositorInsertsYFP-mAID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and mAIDExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sg290860-6
Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PP-MAPK2 (3RP9)
Plasmid#32862PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK2
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acids 123-548 only, P421LPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pGCaMP5
Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
UseTagsExpressionMutationPromoterSAG1 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailabilityAcademic Institutions and Nonprofits only -
pmCherry.LIC.HXGPRT
Plasmid#83364PurposePlasmid contains LIC cassette upstream of an in-frame copy of mCherry and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertmCherry
UseToxoplasma gondiiTagsmCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ptdTomato.LIC.HXGPRT
Plasmid#83365PurposePlasmid contains LIC cassette upstream of an in-frame copy of tdTomato and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInserttdTomato
UseToxoplasma gondiiTagstdTomatoExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFP.HXGPRT
Plasmid#83366PurposePlasmid contains LIC cassette upstream of an in-frame copy of CFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertCFP
UseToxoplasma gondiiTagsCFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailabilityAcademic Institutions and Nonprofits only -
3HX4
Plasmid#60137PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTgCDPK4:MAC03F-A01:C208088
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationSee commentsPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.DHFR
Plasmid#83114PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and DHFR-TS selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.HXGPRT
Plasmid#83128PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsYFPExpressionMutationPromoterEndogenous GeneAvailabilityAcademic Institutions and Nonprofits only -
pSF-TAP.LIC.HXGPRT
Plasmid#83367PurposePlasmid contains LIC cassette upstream of an in-frame copy of SF-TAP and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertStrep-FLAG
UseToxoplasma gondiiTagsSF-TAPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRTagsExpressionMutationPromoterDHFR-TS 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
ATSR
Plasmid#50052Purposeexpresses t.gondii VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-ATSR
UseTagsHISExpressionMammalianMutationS158APromoterChick beta-actin promoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-UPRT
Plasmid#69310Purposeexpresses UPRT cDNADepositorInsertUPRT cDNA
UseTagsHAExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT
Plasmid#87260PurposeYFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.DepositorInsertsYFP-AID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and AIDExpressionMutationPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 112 results