Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pNKY70
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4400
- Notes
- Contains a HIS4C/URA3 fusion cassette that can be used to make fusions to other proteins of interest. Also includes a M13 sequencing primer approximately 300 bp from the HIS4C/URA3 fusion junction. The order of the major features in this plasmid is: M13 primer - ClaI - XhoI - SalI - XbaI (stop) - BamHI - EcoRI - HIS4C bridge/URA3 - BamHI - HindIII - BglII - NcoI - EcoRI - M13 ori - pMB1 ori - ampR. Encodes 93 amino acids of HIS4C and the complete 267 amino acids of URA3. Constructed from pNKY48 (ATCC 77182) by inserting an EcoRI 8-mer at the HindIII site in the polylinker. Restriction digests of the clone give the following sizes (kb): BglII--4.4; EcoRI--2.9, 1.5; PstI--4.4; BamHI--3.2, 1.2; XbaI--4.4. (ATCC staff) Can be used for promoter/enhancer trap screens. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae DBY745, Saccharomyces cerevisiae SK1, Saccharomyces cerevisiae S288C. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77183
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified