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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pLS88

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4772
Notes
Broad host range plasmid containing three antibiotic resistance markers. Natural isolate from Haemophilus ducreyi CH37. The sulfonamide resistance gene (sulII) contains PstI and HincII sites. The kanamycin resistance gene contains unique sites for ClaI, HindIII, PvuI, SmaI and XhoI. The order of the major features of the plasmid is: PstI - P6 promoter - sulII - strA --> - oriV - kanR --> - P5 promoter. [5] Demonstrated to transform (electroporation) Enterobacteriaceae, Pasteurellaceae, and Pseudomonaceae, but not Camplyobacter, Streptococcaceae, or Staphylococcus aureus (ATCC 25923). [3] Growth media: LB plus kanamycin (50 ug/ml) (ATCC number 1236) 37C Deposited by: W.L.Albritton Hosts: broad host range, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
86980
GenBank
L23118
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified