Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pLA2920
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 20000
- Notes
- This vector transfers efficiently to a broad host range including E.coli HB101, Methylobacterium organophilum DSM 761, Pseudomonas AM1, Pseudomonas putida, and Pseudomonas aeruginosa. This was constructed from pLA2917 (ATCC 39840) by deleting the kanamycin resistance segment from Tn5 to avoid internal rearrangement when the transposon moves and to permit selection of conjugants that have a Tn5 insertion. This is a cosmid with a broad host range that can be used to characterize the genome of C1-utilizing microorganisms. Restriction digests of the clone give the following sizes (kb): PstI--20.0; HindIII--20.0; BglII--20.0; EcoRI--17.0; 2.6. (ATCC staff) Medium is 1273 LB plus tetracycline. Hosts: E.coli HB101, broad host range, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 39841
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified