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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pIJ702

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5650
Notes
The tyrosinase (mel) gene of S. antibioticus was inserted at a BclI site of pIJ350, a non-conjugative broad host range vector. Tyrosinase is secreted during growth of S. antibioticus but remains intracellular in S. lividans. Inserts at BglII, SphI or SstI sites inactivate the tyrosinase gene. Cloning vector with thiostrepton resistance (tsrR) and tyrosinase production (mel+) markers, for use in Streptomyces lividans. Medium is 1339 R2 YE medium. Hosts: Streptomyces lividans 3131, Streptomyces lividans. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
35287
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified