Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCZ105
Information
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Notes
- E.coli trp promoter DNA from pHI7. bovine cDNA provided by J.D.Baxter, grown in E.coli K-12 (RV308 cells), clone pCZ105. [1] constructed expression plasmids to get more efficient production of growth hormone (bGH). Besides an efficient promoter (trp) additional codons in the 5' end of the growth hormone coding sequence were required to raise the production level. An artificial sequence (bp 22-97) was inserted, between the TaqI and HgiAI sites of which the first eight codons after the bGH start codon were foreign to the natural gene. When the number of artificial codons just 3' of the 'atg' codon was decreased, the amount of growth hormone produced also decreased dramatically. Bases 30-53 are missing in pCZ110, and replaced by 'gat' and 'gatgataag' in pCZ115 and pCZ112, respectively. Variations, as annotated in the Sites Table, also caused different levels of bGH production. NCBI gi: 208981 Hosts: E.coli. Related vectors: pHI7, pCZ110, pCZ115, pCZ112. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified