Showing: 1 - 20 of 1613 results
-
Plasmid#154824Purpose(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegansDepositorInsertPeft-3::wrmScarlet::tbb-2 UTR
UseTagsExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pMS4 (Empty insertion vector with SEC)
Plasmid#154837PurposeVector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selectionDepositorInsertSEC
UseCRISPR and Cre/LoxTagsExpressionWormMutationsqt-1(e1350)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)
Plasmid#154838PurposeInsertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selectionDepositorInsertsynthetic guide::∆HYGR::unc-54 3' UTR
UseCRISPR and Cre/LoxTagsExpressionWormMutation∆HYGR is promoterless and encodes aa 60-341PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMS79 (eft-3p::Cas9 + sgRNA)
Plasmid#154839PurposeCas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGGDepositorInsertsgRNA
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxTagsExpressionWormMutationHYGR∆ encodes aa 1-226PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSF11-wNIR-GECO2-T2A-HO1
Plasmid#159606PurposeCodon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmidDepositorInsertwNIR-GECO2-T2A-HO1
UseTagsExpressionWormMutationPromotertag-168AvailabilityAcademic Institutions and Nonprofits only -
pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
Plasmid#159794PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertpEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
Plasmid#159795PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertpEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]
Plasmid#159796PurposeHistamine based negative selection marker to paralyze animals with arraysDepositorInsertpEXP[Prpl-3 | histamine | rpl-3 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]
Plasmid#159797PurposeHistamine based negative selection marker to paralyze animals with arraysDepositorInsertpEXP[Pmlc-2 | histamine | rpl-3 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Plasmid#159798PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Plasmid#159799PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
Plasmid#159800PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Plasmid#159801PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
Plasmid#159803PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1358 - Intron GG1 - smu-1
Plasmid#159804PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1359 - Intron GG2 - smu-1
Plasmid#159805PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1360 - Intron GG3 - smu-1
Plasmid#159806PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
Plasmid#159807PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 1613 results