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Plasmid#183652PurposePtdIns(3,4,5)P3 biosensorDepositorInsertBTK (BTK Frog, Human)
UseTagsX. laevis map2k1.L(32-44)-EGFPExpressionMammalianMutationAmino acids 2-174 and 2-170PromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
UseTagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXL010-Wnt dual (GFP-Fire) reporter
Plasmid#40588DepositorInsertWnt reporter sequences
UseLentiviralTagsExpressionMammalianMutationPromoterWnt promoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
KSP_K560GFP
Plasmid#129770PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKSP (kif11.L Frog)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tg(mpeg1:LifeAct-mRuby)
Plasmid#166977Purposelabels F-actin in macrophagesDepositorUseTagsLifeact-mRuby and mRubyExpressionMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG NBT:NRGIIIa:polyA
Plasmid#193012PurposeTol2 transgenic construct that will express human Neuregulin1 Type IIIa in neuronsDepositorInsertsUseTransposon transgenesisTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-XTbx5-GR
Plasmid#117248PurposemRNA synthesisDepositorInsertTbx5-GR (tbx5-b Frog)
UseXenopus expressionTagsExpressionMutationchimeric protein with human glucocorticoid recep…PromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVTRup
Plasmid#101362PurposeHighly efficient in vitro transcription. Empty backbone.DepositorInsert5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
UseFor cloning a gene for in vitro transcription pur…TagsExpressionMutationPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-PH-BTKx2
Plasmid#183654PurposePtdIns(3,4,5)P3 biosensorDepositorInsertBTK (BTK Frog, Human)
UseTagsX. laevis map2k1.L(32-44)-mCherryExpressionMammalianMutationAmino acids 2-174 and 2-170PromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Lunatic Fringe
Plasmid#24962DepositorInsertLunatic Fringe (lfng Frog)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_Zeo
Plasmid#118596PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON
Plasmid#208797PurposeXenopus laevis His6-Myc-DONSON wild typeDepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
Plasmid#105127PurposeUsed along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent proteinDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromotercryaa (crystallin) and fabp10aAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
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XE57 Delta N-cadherin
Plasmid#17025DepositorInsertdominant N-cadherin
UseXenopus expressionTagsExpressionMutationnt 90-2004 deletedPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pCS2+ centrin-RFP
Plasmid#26753DepositorInsertCentrin 4 (cetn4 Frog)
UseReporter for basal bodyTagsmonomeric RFPExpressionMutationPromoterAvailable sinceFeb. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_puromycin
Plasmid#118595PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 xenopus sizzled
Plasmid#24684DepositorInsertSizzled (szl Frog)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2010AvailabilityAcademic Institutions and Nonprofits only