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142,818 results

Showing: 1 - 20 of 142818 results

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pCDNA-hNgAgo

Plasmid

#104384

Purpose

human codon optimized NgAgo; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. do

Insert

hNgAgo

Use

Tags

3XFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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PB-eDIO-ChR2-EYFP

Plasmid

#104387

Purpose

PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

Depositor

Volker Busskamp

Article

Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

Insert

Channelrhodopsin 2

Use

Cre/Lox; Piggybac

Tags

EYFP

Expression

Mammalian

Mutation

E134R

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-eDIO-EYFP

Plasmid

#104388

Purpose

PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

Depositor

Volker Busskamp

Article

Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

Insert

Enhanced Yellow Fluorescent Protein

Use

Cre/Lox; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET246-pUC57 12xMS2V6

Plasmid

#104390

Purpose

MS2V6 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET251-pUC 12xMS2V6 Loxp KANr Loxp

Plasmid

#104392

Purpose

Yeast endogenous mRNA tagging

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-YY1

Plasmid

#104395

Purpose

HA-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1 (YY1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLAG-YY1

Plasmid

#104396

Purpose

FLAG-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1

Use

Tags

FLAG

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-Cas9/CD4-TK2

Plasmid

#104397

Purpose

The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

Depositor

Jeronimo Blanco

Article

Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

Insert

TK2 (TK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
PVS10

Plasmid

#104398

Purpose

polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
PIA586

Plasmid

#104399

Purpose

vector for expression of single E.coli RNAP subunit

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoD

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
PIA900

Plasmid

#104401

Purpose

Polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA, B, C, and Z

Use

E. coli expression vector

Tags

encodes a C-terminally His-tagged rpoC which can …

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3/TO/CD9-GFP

Plasmid

#104402

Purpose

Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

Depositor

Jens Gruber

Article

Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

Insert

CD9 (CD9 Human)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p-dCas9-DNMT3a-Hygro

Plasmid

#104403

Purpose

transient expression of dCas9-DNMT3a fusion protein

Depositor

Stephan Beck

Insert

DNMT3a (DNMT3A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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p-dCas9-TET1-Hygro

Plasmid

#104404

Purpose

transient expression of dCas9-TET1 fusion protein

Depositor

Stephan Beck

Insert

TET1 (TET1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
p-dCas9-VP64-Hygro

Plasmid

#104405

Purpose

transient expression of dCas9-VP64 fusion protein

Depositor

Stephan Beck

Insert

VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
p-dCas9-LSD1-Hygro

Plasmid

#104406

Purpose

transient expression of dCas9-LSD1 fusion protein

Depositor

Stephan Beck

Insert

LSD1 (KDM1A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

IRATp970A0364D (Source Bioscience) has a P405H ch…

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
p-dCas9-SET7-Hygro

Plasmid

#104407

Purpose

transient expression of dCas9-SET7 fusion protein

Depositor

Stephan Beck

Insert

SET7 (SETD7 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge