pBABE-puro
(Plasmid #1764)

• Purpose: (Empty Backbone) Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.
• Depositing Labs: Hartmut Land, Jay Morgenstern, Bob Weinberg
• Publication: Morgenstern et al Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.

Backbone

• Vector backbone: pBABE-puro
• Backbone size (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Supplemental Documents:
  • pBABE protocol
• Articles Citing this Plasmid:
  • 229 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.