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CRISPR Pooled Libraries

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CRISPR Resources

CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target cells are treated with the pooled library to create a population of mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus.

Before ordering, please review the steps for using pooled CRISPR libraries and keep the following in mind:

  • CRISPR screening experiments require electroporation to amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation.
  • CRISPR screening experiments require large quantities of cells and therefore, may not be suitable for use when the number of cells is limited (e.g. primary cells).
  • Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped and authorized to use lentivirus in your lab.

Once you have determined that CRISPR-based screening is appropriate for your experiment, the next step is to pick a library. The pooled CRISPR libraries in Addgene’s collection differ based on some important criteria, and it is important to know which library best suits your particular experiment. Some important variables include:

  • Type of genetic modification - CRISPR libraries vary based on whether they are used to knockout, activate or repress target genes.
  • Number of gRNAs - A given CRISPR library may target the entire genome or a specific class of genes (subpools).
  • Species - the gRNAs within a given CRISPR library are unique to the genome of particular organism, and the library is only compatible with cells derived from that organism.

All of our current pooled CRISPR libraries can be found in the following table, which also includes information about the type of genetic modification and target species. Additional information about each library can be found on the individual library page.

Find Pooled Libraries of Interest

Click on different properties to create a custom filtered list of the CRISPR pooled libraries in Addgene's collection.

Library Type
Species
Library Size
Viral Prep Available
Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs
Adamson DNA Repair CRISPRi Libraries 177663, 177664, 177670 Inhibition Human Adamson 3rd ∼3 366 or 1,573
Activity-optimized genome-wide library Discontinued Knockout Human Sabatini and Lander 3rd 10 178,896
Activity-optimized genome-wide library 1000000100 Knockout Human Sabatini and Lander 3rd 10 187,535
Advanced Catalogue of Epigenetic Regulators (ACER) 226117 Knockout Human Lu 3rd 10 8,205
Bassik Human CRISPR Knockout Library 101926 — 101934 Knockout Human Bassik 3rd 10 Varies
Bassik Mouse CRISPR Knockout Library 1000000121 — 1000000130 Knockout Mouse Bassik 3rd 10 Varies
Berger Lab Human Paralog Knockout Library (pgPEN) 171172 Knockout Human Berger 3rd 8-16 33,170
Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919
Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ∼5 21,417
Bison sgRNA Library 169942 Knockout Human Ebert 3rd 4 2,852
Bonifacino Lab Human ubiquitination-related proteins CRISPR KO library 174592 Knockout Human Bonifacino 3rd 6-25 11,108
Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina N/A 1 Varies
Bovine CRISPR Knockout Libraries 213927, 213928 Knockout Cow Tan 2nd 4 on average 94,000
Bradley Human CRISPR Poison Exon Knockout Library 138084 Knockout Human Bradley 3rd ~9 pairs/exon 9,508
Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout Mouse Bradley 3rd 8-10 pgRNAs 1,718
Broad GPP genome-wide Brunello 73179 (1 plasmid)
73178 (2 plasmid) 		 Knockout Human Doench and Root 3rd 4 76,441
Broad GPP genome-wide Brie 73632 (1 plasmid)
73633 (2 plasmid) 		 Knockout Mouse Doench and Root 3rd 4 78,637
Broad GPP Humagne Set C and Humagne Set D 172650 (Set C)
172651 (Set D)		 Knockout Human Doench and Root 2nd 2 40,710
Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551
209552		 Knockout Human Doench 3rd 1, 2, or 4 49,766 arrays
Broad GPP kinome Brunello 75314, 75315
(1 plasmid)
75312, 75313
(2 plasmid)		 Knockout Human Doench and Root 3rd 4 3,052
Broad GPP kinome Brie 75317 (1 plasmid)
75316 (2 plasmid)		 Knockout Mouse Doench and Root 3rd 4 2,852
Broad GPP activation Calabrese p65-HSF 92379 (Set A) 
92380 (Set B) 		 Activation Human Doench and Root 3rd 3–6 56,762 (Set A)
56,476 (Set B)
Broad GPP activation Caprano p65-HSF 92383 (Set A)
92384 (Set B)		 Activation Mouse Doench and Root 3rd 3–6 67,187 (Set A)
66,889 (Set B)
Broad GPP inhibition Dolcetto 92385 (Set A)
92386 (Set B)		 Inhibition Human Doench and Root 3rd 3–6 57,050 (Set A)
57,011 (Set B)
Broad GPP inhibition Dolomiti 104090 (Set A)
104091 (Set B)		 Inhibition Mouse Doench and Root 3rd 3–6 67,366 (Set A)
67,194 (Set B)
Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang N/A N/A N/A - The protospacers contained in the library represent all 4096 (46) combinations of 6 nucleotides.
Chong Zhang E. coli Genome-wide Inhibition Library 113134 — 113138
113146, 113147		 Inhibition E. coli Chong Zhang N/A 15 Varies
CRiNCL - Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies
CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library 82480 Knockout Mouse Xie N/A N/A 395
CRISPRa Discontinued Activation Human Weissman 3rd 10 198,810
CRISPRa-v2 83978
1000000091		 Activation Human Weissman 3rd 5
10		 104,540
209,080
CRISPRa-v2 83996
1000000093		 Activation Mouse Weissman 3rd 5
10		 107,105
214,210
CRISPRi Discontinued Inhibition Human Weissman 3rd 10 206,421
CRISPRi-v2 83969
1000000090		 Inhibition Human Weissman 3rd 5
10		 104,535
209,070
CRISPRi-v2 83987
1000000092		 Inhibition Mouse Weissman 3rd 5
10		 107,415
214,830
DNA Damage Response MKOv4 Library 140219 Knockout Human J. Chen 3rd 10 4,530
Murine Cell Surface Protein Knockout (McspKO) Library 200445 Knockout Mouse J. Chen 3rd 3-4 5,001
Drosophila Cell CRISPR Knockout Library 134582
134583
134584		 Knockout Fly Perrimon 3rd 8
6
6		 7,956
17,827
59,406
Dual sgRNA CRISPRi Libraries 187246 — 187248
1000000192		 Inhibition Human Weissman 3rd 6 Varies
Dual sgRNA CRISPRa Libraries 187249 — 187251
1000000193		 Activation Human Weissman 3rd 6 Varies
Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini and Lander 3rd 10 Varies
FNLCR CRISPRa Cell Surface Libraries 207471
1000000228		 Activation Human Chari 3rd 5 30,868
35,517
Focused Ras Synthetic Lethal Human CRISPR Knockout Library 92352 Knockout Human Sabatini and Lander 3rd 50 6,661
Garnett Lab MinLibCas9 Library 164896 Knockout Human Garnett 3rd 2 37,722
Green monkey (Chlorocebus sabaeus) sgRNA library 178284 Knockout Green Monkey Doench and Root 3rd 4 84,963
Human CRISPR Knockout Pooled Library (Gattinara) 136986 Knockout Human Doench 3rd 2 40,964
Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition Yeast Ingolia NA ~6 44,580
KSHV Tiling Library 180272 Knockout KSHV Glaunsinger NA NA 24,000
BLaER1 pgRNA Library 183825 Knockout Human Guigo 3rd 10 11,750
Mouse Cherry Brie Pooled Library 170511 Knockout Mouse Tolar 3rd ~4 78,637
Mouse Chromatin Regulator Library 200011 Knockout Mouse Griffin, Manguso, and Bernstein 3rd 6 ~5616
Mouse CRISPR Knockout Pooled Library (Gouda) 136987 Knockout Mouse Doench 3rd 2 44,155
hCRISPRa-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies
hCRISPRi-v2 subpooled libraries 83971 — 83977 Inhibition Human Weissman 3rd 5 Varies
mCRISPRa-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies
mCRISPRi-v2 subpooled libraries 83989 — 83995 Inhibition Mouse Weissman 3rd 5 Varies
Human CRISPRi titration libraries 136478 & 136479 Inhibition Human Weissman 3rd Varies Varies
Human CRISPRi-v2 Dual Guide Libraries 197348
197349		 Inhibition Human Voorhees 3rd 5 103,269
Human CRISPR Knockout Library 1000000132 Knockout Human X.S. Liu 3rd 10 185,634
Human Epigenetic Knockout Library 162256 Knockout Human Birsoy 3rd 8 20,051
Human GeCKO v2 1000000048
(1 plasmid)
1000000049
(2 plasmid)		 Knockout Human Zhang 3rd 6 123,411
Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406
Human improved genome-wide library v1 67989 Knockout Human Yusa 3rd 5 90,709
Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman
OhAinle		 3rd 8 15,416
Human CRISPR lncRNA Activation Pooled Library 1000000106 Activation Human Zhang 3rd 10 96,458
Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini 3rd 10 30,290
Human DNA Binding Domain-Focused CRISPR Knockout Library 123334 Knockout Human Vakoc 3rd 6 8,658
Human Genome-Wide Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576
Human Whole Genome sgRNA iBAR Library 140633 Knockout Human Wei 3rd 3 58,630
Human Kinase Domain-Focused CRISPR Knockout Library 117725 Knockout Human Vakoc 3rd 6 3,051
Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann 3rd ~10 13,920
Human lncRNA Splicing-targeting CRISPR Library 119977 Knockout Human Wei 3rd Varies 126,773
Human mTORC1 Focused Library 164084 Knockout Human Sabatini 3rd 10 7,501
Human Membrane Protein Activation Library 113345 Activation Human Wright 3rd 7-14 58,570
Human miRNA CRISPR Knockout Library 112200 Knockout Human Lin 3rd 4-5 8,382
Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs (lncRNAs) 89640 Knockout Human Wei 3rd Varies 12,472 pairs
Human SLC Activation Library 132561 Activation Human Superti-Furga 3rd 6 3,342
Human SLC Knockout Library 132552 Knockout Human Superti-Furga 3rd 6 2,609
Human Transporter Knockout Libraries 213695
221409
221410		 Knockout Human Superti-Furga 3rd Varies Varies
Human Transcription Factor Knockout Library 162275 Knockout Human Li 3rd ~7 11,364
Dow Lab Base Editing Sensor Libraries 179217-179219 Base Editing Human, Mouse Dow 3rd ~100 Varies
Mini-human AsCpf1-based Human Genome-wide Knockout Library 130630 Knockout Human Draetta 3rd 3-4 17,032 arrays
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout Mouse Pu N/A 6 14,671
Pan-cancer CasRx lncRNA Albarossa Library 212966 Knockout Human Rad 3rd Varies 156,250
Mouse CRISPR Knockout Library in lentiCRISPR v2-Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836
Mouse GeCKO v2 1000000052
(1 plasmid)
1000000053
(2 plasmid)		 Knockout Mouse Zhang 3rd 6 130,209
Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897
Mouse improved genome-wide library v2 67988 Knockout Mouse Yusa 3rd 5 90,230
Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248
206249		 Knockout Mouse Birsoy 3rd 7–10 2,105
630
Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy 3rd 8 22,909
Mouse Liver CRISPR Knockout Library 192824 Knockout Mouse Knouse 3rd ~5 71,878
Mouse messenger-RBP sgRNA Library 169082 Knockout Mouse Turner N/A 10 13,500
Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361 Knockout Mouse Chen 3rd 4 11,934 arrays
Mouse SLC Knockout Library 141436 Knockout Mouse Superti-Furga 3rd 6 2,384
Mouse Toronto KnockOut (mTKO) CRISPR Library 159392
159393		 Knockout Mouse Moffat 3rd ~5 19,463
Mouse Transcription Factor Activation Library 122131 Activation Mouse Qi 3rd 20 51,762
Mouse Tumor Suppressor Gene CRISPR Knockout Library 113584 (EFS)
113585 (TBG)		 Knockout Mouse Chen N/A (AAV) 4 286
Mouse Validation (mVAL) CRISPR Library 159391 Knockout Mouse Moffat 3rd 4 182
Mycobacterium tuberculosis CRISPRi Library (RLC12) 163954 Inhibition M. tuberculosis Rock NA Varies 96,700
Mycobacterium smegmatis CRISPRi Library (RLC11) 163955 Inhibition M. smegmatis Rock NA Varies 176,286
Neelamegham Human GlycoGene CRISPR Library 140961 Knockout Human Neelamegham 3rd 10 3,637
Oxford Fly 64750 Knockout D. melanogaster Liu N/A 3 40,279
Pan-Druggable Cancer Library 182133 Knockout Human Mali 3rd 1 74
Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A
Retroviral Mouse Genome-wide CRISPR Knockout Library 104861 Knockout Mouse Teichmann N/A (retroviral) 5 90,230
RNA-Binding Protein Pooled CRISPR Knockout Library 141438 Knockout Human Yeo 3rd 10 12,472
SAM v1 - 3 plasmid system 1000000057 (Zeocin)
1000000074 (Puromycin)		 Activation Human Zhang 3rd 3 70,290
SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716
SAM v2 - 2 plasmid system 1000000078 (Blasticidin) Activation Human Zhang 3rd 3 70,290
CHyMErA Paralog & Dual-targeting hgRNA pooled library 155199 Knockout Human Moffat
Blencowe		 3rd variable 92,746
CHyMErA Exon-deletion hgRNA pooled library 155200 Knockout Human Moffat
Blencowe		 3rd variable 91,948
Toronto KnockOut - Version 1 1000000069 Knockout Human Moffat 3rd 12 176,500
Toronto KnockOut - Version 3 90294 (1 plasmid)
125517 (2 plasmid)		 Knockout Human Moffat 3rd 4 70,948
Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A 10 8,158
Turner Lab Human messenger-RBP sgRNA Library 168791 Knockout Human Turner 3rd 10 8,260
Two plasmid human activity-optimized genome-wide library 1000000095 Knockout Human Sabatini and Lander 3rd 10 187,536
Two plasmid mouse activity-optimized genome-wide library 1000000096 Knockout Mouse Sabatini and Lander 3rd 10 188,509
Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast Tavazoie N/A 6-12 51,681
Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~6 18,343
Human UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274
MYC-CRISPR Library 173195 Knockout Human Dzikiewicz-Krawczyk 3rd 1-7 per element 46,354
BARBEKO sgRNA Library 174163 Knockout, Base Editing Human Wei 3rd 3 53,502
sgRNA Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening N/A 1 1,499
MusCK Library 174196 (Library A)
174197 (Library B)
1000000178 (A & B)		 Knockout Mouse X.S. Liu 3rd 5
5
10		 49,252
Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation Human Birsoy 3rd Varies 32,460
Mouse Metabolic Gene CRISPRa sgRNA Library 187081 Activation Mouse Birsoy 3rd Varies 17,031
The Human Druggable CRISPR Library 200013 Knockout Human L. Liu 3rd 5 10,000

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