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Addgene

CRISPR Plasmids and Resources

CRISPR technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are up-to-date with reagents and information you need to use CRISPR in your own lab. Check out our CRISPR resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and Xenopus.

Addgene CRISPR Resources

  • CRISPR Guide: Essential background information on CRISPR and the basics for planning your first CRISPR experiment.
  • CRISPR 101 eBook: Based on our popular CRISPR 101 blog series, we've organized a comprehensive CRISPR resource for you to download.
  • CRISPR Resources: Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and more.
  • CRISPR History: Learn how CRISPR was modified from a bacterial immune system to a revolutionary tool for genome engineering.
  • CRISPR Topic Page: Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's blog.
  • Addgene's Viral Service: Request ready-to-use viral preps of select CRISPR lentiviral plasmids.
  • CRISPR Plasmid Kits: Find groups of plasmids that are used together in CRISPR experiments.
  • CRISPR Pooled Libraries: Find CRISPR libraries for knockout, activation, inhibition, and barcoding experiments.

Browse CRISPR Plasmids by Function

Genome Engineering

Cut
Wild-type Cas enzymes generate double strand breaks (DSBs).
Nick
Mutated Cas nickase enzymes generate single-strand DNA breaks.
Prime Edit
Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific DNA site.
Base Edit
Base editors precisely edit DNA without promoting DSB formation.
DeleteNEW
Cascade-Cas3 generates large genomic deletions.
InsertNEW
CRISPR transposases insert large fragments of DNA.

Transcriptional Regulation

Activate
dCas9 fused to an activator peptide activates gene expression.
Interfere
dCas9 alone, or fused to a repressor peptide, inhibits transcription.
Epigenetics
dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression.

RNA Targeting

RNA Targeting
Type VI CRISPR systems target RNA, not DNA, across multiple systems.
RNA Editing
Cas13 enzymes fused to deaminases can edit bases in RNA.

Other Applications

Screen
CRISPR libraries are a powerful tool for genome-wide screening.
Purify
dCas9 fused to an epitope tag can be used to isolate a given genomic locus.
Visualize
CRISPR fluorescence methods can be used to visualize genomic loci.

gRNAs

Empty gRNA Vectors
Select an empty vector for your experiment based on a variety of factors.
gRNAs
gRNA plasmids to target specific genes.