Charles Gersbach Lab Materials

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Title	Authors	Publication
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.	Polstein LR, Gersbach CA	Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
Activation of the imprinted Prader-Willi syndrome locus by CRISPR-based epigenome editing.	Rohm D, Black JB, McCutcheon SR, Barrera A, Berry SS, Morone DJ, Nuttle X, de Esch CE, Tai DJC, Talkowski ME, Iglesias N, Gersbach CA	Cell Genom. 2025 Feb 12;5(2):100770. doi: 10.1016/j.xgen.2025.100770.
Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.	Liu S, Hamilton MC, Cowart T, Barrera A, Bounds LR, Nelson AC, Dornbaum SF, Riley JW, Doty RW, Allen AS, Crawford GE, Majoros WH, Gersbach CA	Cell Genom. 2025 Feb 4:100766. doi: 10.1016/j.xgen.2025.100766.
CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.	Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA	Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain.	Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak F, Reddy TE, Gersbach CA	ACS Synth Biol. 2014 Dec 10.
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.	Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA	Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases.	Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA	Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.
Gersbach Lab Cas9 Plasmids 2024	(unpublished)
Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.	Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA	Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors.	Polstein LR, Gersbach CA	J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. Epub 2012 Sep 27.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.489.518748.47108	47108	pSPgRNA	SPgRNA	Mammalian Expression, CRISPR	RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
100.92.002249.71236	71236	pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro	humanized dCas9-KRAB T2A Puro, sgRNA	Lentiviral, CRISPR	Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
100.73.712827.71237	71237	pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP	humanized dCas9-KRAB T2A GFP, sgRNA	Lentiviral, CRISPR	Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
100.51.654864.47107	47107	pcDNA-dCas9-VP64	dCas9-VP64	Mammalian Expression, CRISPR	RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
100.46.947386.53188	53188	phU6-gRNA		Mammalian Expression, CRISPR	Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749.
100.28.214089.61357	61357	pcDNA-dCas9-p300 Core	S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)	Mammalian Expression, CRISPR, Synthetic Biology	Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
100.27.858202.53187	53187	pmU6-gRNA		Mammalian Expression, CRISPR	Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749.
100.25.870792.53189	53189	ph7SK-gRNA		Mammalian Expression, CRISPR	Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749.
100.25.668524.53186	53186	phH1-gRNA		Mammalian Expression, CRISPR	Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749.
100.22.251836.53192	53192	pLV hUbC-dCas9 VP64-T2A-GFP	humanized dead Cas9 VP64 T2A GFP	Mammalian Expression, Lentiviral, CRISPR	Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749.