Skip to main content
Addgene

Mouse genome engineering using designer nucleases.

Hermann M, Cermak T, Voytas DF, Pelczar P
J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
37184pCAG-T7-TALEN(Sangamo)-DestinationDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
40131pCAG-T7-TALEN(Sangamo)-FokI-KKR-DestinationDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
40132pCAG-T7-TALEN(Sangamo)-FokI-ELD-DestinationDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

Antibodies from Article