Mouse genome engineering using designer nucleases.
Hermann M, Cermak T, Voytas DF, Pelczar P
J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
37184 | pCAG-T7-TALEN(Sangamo)-Destination | Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains. |
40131 | pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination | Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR). |
40132 | pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination | Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD). |