Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.

Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM, Sheen J
Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
52254	HBT-pcoCas9	transient expression of pcoCas9 gene in plant cells
52255	pUC119-gRNA	Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.