Genetic screens in human cells using the CRISPR-Cas9 system.
Wang T, Wei JJ, Sabatini DM, Lander ES
Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50661 | pCW-Cas9 | Doxycycline-inducible lentiviral expression of SpCas9 |
| 50662 | pLX-sgRNA | Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol) |
| 51043 | Human Lentiviral sgRNA Library - Unknown Function | Sub-pool of Sabatini/Lander sgRNA library enriched for gene targets of unknown function. |
| 51044 | Human Lentiviral sgRNA Library - Kinases | Sub-pool of Sabatini/Lander sgRNA library enriched for kinase targets. |
| 51045 | Human Lentiviral sgRNA Library - Ribosomal Proteins | Sub-pool of Sabatini/Lander sgRNA library enriched for ribosomal gene targets. |
| 51046 | Human Lentiviral sgRNA Library - Cell Cycle | Sub-pool of Sabatini/Lander sgRNA library enriched for cell cycle-related targets. |
| 51047 | Human Lentiviral sgRNA Library - Nuclear | Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets. |
| 51048 | Human Lentiviral sgRNA Library - Control | Sub-pool of Sabatini/Lander sgRNA library enriched for control targets. |