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MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor.

Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M
PLoS One. 2013 Dec 2;8(12):e82009. doi: 10.1371/journal.pone.0082009. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
50742pCMVMagFRET-1Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.
50748pCMVMagFRET-1-NLSExpresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.
50756pCMVMagFRET-6Expresses MagFRET-6 in the cytosol of mammalian cells. Weak affinity control FRET sensor for MagFRET-1.
50757pCMVMagFRET-6-NLSExpresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

Antibodies from Article