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Tuning gene expression with synthetic upstream open reading frames.

Ferreira JP, Overton KW, Wang CL
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49218pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49219pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49220pCru5-ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49221pCru5-GGG/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49222pCru5-CCC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49223pCru5-UCU/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49224pCru5-/CGA-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49225pCru5-/UAA-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49226pCru5-/GCCACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49227pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49228pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49229pWPT-ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49230pWPT-GGG/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49231pWPT-CCC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49232pWPT-UCU/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49233pWPT-/CGA-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49234pWPT-/UAA-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
49235pWPT-/GCCACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Antibodies from Article