Tracking genome engineering outcome at individual DNA breakpoints.
Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM
Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
31475 | pCVL SFFV d14GFP Donor | |
31476 | pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt) | |
31477 | pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP | |
31478 | pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP | |
31479 | pCVL SFFV-EF1s HA.NLS.Sce(opt) | |
31480 | pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro | |
31481 | pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP | |
31482 | pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro | |
31483 | pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a | |
31484 | pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP | |
31485 | pRRL SFFV d20GFP.T2A.mTagBFP Donor | |
32627 | pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP | |
32628 | pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP | |
63586 | psPAX2-D64V | psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene. |