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Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes.

Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, Gumper I, Sabatini DD, De Robertis EM
Cell. 2010 Dec 23. 143(7):1136-48. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
27997pCS2 Smad1 EVE GM
29679pCS2 GSK3-RFP
29680pCS2 GSK3-GFP
29681pCS2 DN-GSK3-GFP
29682pCS2 CA-LRP6-GFP
29683pCS2 xDvl-RFP
29684pCS2 stabilized mutant Beta Catenin-GFP
29685pCS2 HRS-RFP
29687pCS2 Flag-JunB
29688DsRed-Rab5-QL
29689pCS2 GFP-GSK3-MAPKGFP reporter protein fused to three GSK3 phosphorylation sites
29690pCS2 GFP-GSK3mut-MAPKGFP reporter protein fused to three mutated GSK3 phosphorylation sites
29691pCS2 GFP-GSK3-MAPK-E3mut

Antibodies from Article