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siRNAs pools generated in Escherichia coli exhibit strong RNA-interference activity against influenza virus genomic sequences.

Villa R, Renzi S, Dotti S, Lucchini F
Virology. 2022 Dec 31;579:38-45. doi: 10.1016/j.virol.2022.12.013. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196606psiCHECK-EGFPDual-Luciferase Reporter vector carrying an EGFP direct sequence
196607psiCHECK-NPDual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction
196608pBAD-P19-NPsiRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat
196609pBAD-P19-EGFPsiRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat
196654pBAD-6xHis-BglIIDerived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site
196655psiCHECK-2-XNSPromega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

Antibodies from Article