A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility.
Sheu SH, Upadhyayula S, Dupuy V, Pang S, Deng F, Wan J, Walpita D, Pasolli HA, Houser J, Sanchez-Martinez S, Brauchi SE, Banala S, Freeman M, Xu CS, Kirchhausen T, Hess HF, Lavis L, Li Y, Chaumont-Dubel S, Clapham DE
Cell. 2022 Sep 1;185(18):3390-3407.e18. doi: 10.1016/j.cell.2022.07.026.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
189613 | pB-Tet-On-Arl13b-RhoA sensor | Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor |
189614 | pB-Tet-On-HTR6-RhoA sensor | Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor |
189615 | pAAV-Syn1-GRAB-HTR6-cilia | Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter |
189616 | pAAV-TPH2-Cre | Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter |
189617 | pAAV-TRE-HTR6-SNAPf-TRIP | Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible |