Skip to main content
Addgene

A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility.

Sheu SH, Upadhyayula S, Dupuy V, Pang S, Deng F, Wan J, Walpita D, Pasolli HA, Houser J, Sanchez-Martinez S, Brauchi SE, Banala S, Freeman M, Xu CS, Kirchhausen T, Hess HF, Lavis L, Li Y, Chaumont-Dubel S, Clapham DE
Cell. 2022 Sep 1;185(18):3390-3407.e18. doi: 10.1016/j.cell.2022.07.026. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
189613pB-Tet-On-Arl13b-RhoA sensorTet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor
189614pB-Tet-On-HTR6-RhoA sensorTet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor
189615pAAV-Syn1-GRAB-HTR6-ciliaExpresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter
189616pAAV-TPH2-CreExpresses Cre recombinase under the control of the tryptophan hydroxylase promoter
189617pAAV-TRE-HTR6-SNAPf-TRIPExpresses cilia-targeted RhoA inhibitory peptide, tet-inducible

Antibodies from Article