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Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo.

Massengill CI, Bayless-Edwards L, Ceballos CC, Cebul ER, Cahill J, Bharadwaj A, Wilson E, Qin M, Whorton MR, Baconguis I, Ye B, Mao T, Zhong H
Nat Methods. 2022 Nov;19(11):1461-1471. doi: 10.1038/s41592-022-01646-5. Epub 2022 Oct 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182279pCDNA3_cAMPFIRE-LMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.
182280pCDNA3_cAMPFIRE-MMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.
182281pCDNA3_cAMPFIRE-HMammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.
182282pCDNA3_cAMPFIRE-L-R279EMammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.
182283pCAGGS_cAMPFIRE-LMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.
182284pCAGGS_cAMPFIRE-MMammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.
182285pCAGGS_cAMPFIRE-HMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.
182286pCAGGS_cAMPFIRE-L-R279EMammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Antibodies from Article