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Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.

Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR
Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174817pCMV-SaPE2Mammalian expression of SaCas9 prime editor 2
174818pCMV-SaPE2-P2A-BSDMammalian expression of SaCas9 prime editor 2 with P2A-BSD marker
174819pCMV-PE2-P2A-BSDMammalian expression of SpCas9 prime editor 2 with P2A-BSD marker
174820pCMV-PEmaxMammalian expression of SpCas9 PEmax prime editor
174821pCMV-PEmax-P2A-BSDMammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker
174823pEF1a-hMLH1dn (og codon)Mammalian expression of human MLH1dn (original codon usage)
174824pEF1a-hMLH1dnMammalian expression of human MLH1dn (codon optimized)
174825pEF1a-mMLH1dnMammalian expression of mouse MLH1dn (codon optimized)
174826pEF1a-hMLH1NTD-NLSMammalian expression of human MLH1NTD-NLS (codon optimized)
174827pCMV-PE2-P2A-hMLH1dnMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)
174828pCMV-PEmax-P2A-hMLH1dnMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
174829pPC1000Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
177670DNA repair library with 1,513 sgRNAsCRISPR-interference (CRISPRi) library designed to target 476 genes involved in DNA repair and associated processes. It contains 1,513 sgRNAs and 60 non-targeting control sgRNAs.
178113pT7-PEmax for IVTTemplate for in vitro transcription of PEmax
178114pT7-hMLH1dn for IVTTemplate for in vitro transcription of human MLH1dn
180020pCMV-PEmax-P2A-GFPMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Antibodies from Article