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Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion.

Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Alves Dias R, Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de Mendonca JP, Azeloglu EU, Zou H, Friedel RH
Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
98398pLKO-Tet-On-shRNA-ControlLentivirus for expression of non-targeting control shRNA (Dox-inducible)
98399pLKO-Tet-On-PLXNB2-shRNA1Lentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)
98400pLKO-Tet-On-PLXNB2-shRNA2Lentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)
156166pLenti-PGK-RAP1A-WT-puroLentiviral vector for expression of RAP1A-WT, with puromycin selection
156167pLenti-PGK-RAP1A-E63-puroLentiviral vector for expression of RAP1A-E63, with puromycin selection
156168pLenti-PGK-flag-RAP1B-V12-puroLentiviral vector for expression of flag-RAP1B-V12, with puromycin selection
156169pLenti-PGK-flag-RAP1B-N17-puroLentiviral vector for expression of flag-RAP1B-N17, with puromycin selection
156170pLenti-PGK-RAP2A-WT-puroLentiviral vector for expression of RAP2A-WT, with puromycin selection
156171pLenti-PGK-RAP2A-V12-puroLentiviral vector for expression of RAP2A-V12, with puromycin selection
156172pLenti-PGK-RAP2A-N17-puroLentiviral vector for expression of RAP2A-N17, with puromycin selection
156173pLenti-PGK-RAP1A-WT-neoLentiviral vector for expression of RAP1A-WT, with neomycin section
156174pLenti-PGK-RAP1A-E63-neoLentiviral vector for expression of RAP1A-E63, with neomycin selection
156175pLenti-PGK-flag-RAP1B-V12-neoLentiviral vector for expression of flag-RAP1B-V12, with neomycin selection
156176pLenti-PGK-flag-RAP1B-N17-neoLentiviral vector for expression of flag-RAP1B-N17, with neomycin selection
156177pLenti-PGK-RAP2A-WT-neoLentiviral vector for expression of RAP2A-WT, with neomycin selection
156178pLenti-PGK-RAP2A-V12-neoLentiviral vector for expression of RAP2A-V12, with neomycin selection
156179pLenti-PGK-RAP2A-N17-neoLentiviral vector for expression of RAP2A-N17, with neomycin selection
176846pLKO-Tet-On-YAP1-shRNA1lentiviral vector for doxycycline-inducible human YAP1 knockdown
176847pLKO-Tet-On-YAP1-shRNA2lentiviral vector for doxycycline-inducible human YAP1 knockdown
176848pLV-hPLXNB2_PGK_Neolentiviral vector for constitutive PLXNB2 overexpression
176849pLV-CMVtight-PLXNB2 iOElentiviral vector for doxycycline-inducible PLXNB2 overexpression
176850pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Purolentiviral vector for doxycycline-inducible YAP1 overexpression
176851pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Purolentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Antibodies from Article