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Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration.
Liu F, Kambakam S, Almeida MP, Ming Z, Welker JM, Wierson WA, Schultz-Rogers LE, Ekker SC, Clark KJ, Essner JJ, McGrail M
Elife. 2022 Jun 17;11. pii: 71478. doi: 10.7554/eLife.71478. PubMed Article

Plasmids from Article

ID Plasmid Purpose
173886pUFlip-floxed2A-mRFP-; gcry1:BFP -0Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
173887pUFlip-floxed2A-mRFP-; gcry1:BFP -1Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
173888pUFlip-floxed2A-mRFP-; gcry1:BFP -2Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
173889pUFlip-floxed2A-KalTA4-; gcry1:BFP -0Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
173890pUFlip-floxed2A-KalTA4-; gcry1:BFP -1Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
173891pUFlip-floxed2A-KalTA4-; gcry1:BFP -2Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
180007pUFlip-floxed2A-mRFP-; HE1A:BFP -0Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
180008pUFlip-floxed2A-mRFP-; HE1A:BFP -1Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
180009pUFlip-floxed2A-mRFP-; HE1A:BFP -2Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
180010pUFlip-floxed2A-KalTA4-; HE1A:BFP -0Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
180011pUFlip-floxed2A-KalTA4-; HE1A:BFP -1Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
180012pUFlip-floxed2A-KalTA4-; HE1A:BFP -2Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Antibodies from Article