MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein-protein interactions.
Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, Crete P, Field B
Plant Methods. 2022 May 26;18(1):69. doi: 10.1186/s13007-022-00902-1.
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PubMed
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Article
A subset of these plasmids is included in the MoBiFC toolkit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172774 | pLBC0010 | Level 0 module containing NT1_CTPSSU |
| 172775 | pLBC0011 | Level 0 module containing NT2_CTPSSU |
| 172776 | pLBC0020 | Level 0 module containing NT1_OEP7 |
| 172777 | pLBC0030 | Level 0 module containing NT2_nYFP |
| 172778 | pLBC0031 | Level 0 module containing CT_nYFP |
| 172779 | pLBC0040 | Level 0 module containing NT2_cYFP |
| 172780 | pLBC0041 | Level 0 module containing CT_cYFP |
| 172781 | pLBC0050 | Level 0 module containing NT2_cYFP |
| 172782 | pLBC0051 | Level 0 module containing CT_3XFLAGnYFP |
| 172783 | pLBC0060 | Level 0 module containing NT2_cYFP3XHA |
| 172784 | pLBC0061 | Level 0 module containing CT_cYFP3XHA |
| 172785 | pLBC0071 | Level 0 module containing CT_nVenus |
| 172786 | pLBC0080 | Level 0 module containing NT2_nVenus |
| 172787 | pLBC0081 | Level 0 module containing CT_nVenus |
| 172788 | pLBC0090 | Level 0 module containing NT2_Start |
| 172789 | pLBC0100 | Level 0 module containing CT_Stop |
| 172790 | pLBC0110 | Level 0 module containing CDS1_spacer |
| 172791 | pLBC0120 | Level 0 module containing CDS2ns_mHSP21 |
| 172792 | pLBC0130 | Level 0 module containing CDS2ns_mPTAC5 |
| 172793 | pLBC0131 | Level 0 module containing CDS2ns_deltamPTAC5 |
| 172794 | pLBC0140 | Level 0 module containing CDS2ns_mCHERRY |
| 172795 | pLBC0150 | Level 0 module containing NT2_TurboID |
| 172796 | pLBC0151 | Level 0 module containing CT_TurboID |
| 172797 | pLBT0010 | Level 1 module containing 35S-HSP21-GFP_P2 F |
| 172798 | pLBT0020 | Level 1 module containing 35S-HSP21-nYFP_notag_P2 F |
| 172799 | pLBT0030 | Level 1 module containing 35S-HSP21-cYFP_notag_P3 F |
| 172800 | pLBT0040 | Level 1 module containing 35S-HSP21-nYFP_P2 F |
| 172801 | pLBT0050 | Level 1 module containing 35S-HSP21-cYFP_P3 F |
| 172802 | pLBT0060 | Level 1 module containing 35S-nYFP-HSP21_P2 F |
| 172803 | pLBT0070 | Level 1 module containing 35S-cYFP-HSP21_P3 F |
| 172804 | pLBT0080 | Level 1 module containing 35S-HSP21-nVENUS_P2 F |
| 172805 | pLBT0090 | Level 1 module containing 35S-HSP21-cVENUS_P3 F |
| 172806 | pLBT0100 | Level 1 module containing 35S-PTAC5-cYFP_P3 F |
| 172807 | pLBT0110 | Level 1 module containing 35S-deltaPTAC5-cYFP_P3 F |
| 172808 | pLBT0120 | Level 1 module containing 35S-CTP-mCHERRY-nYFP_P2 F |
| 172809 | pLBT0130 | Level 1 module containing 35S-CTP-mCHERRY-cYFP_P3 F |
| 172810 | pLBT0140 | Level 1 module containing 35S-CTP-mCHERRY-nVENUS_P2 F |
| 172811 | pLBT0150 | Level 1 module containing 35S-CTP-mCHERRY-cVENUS_P3 F |
| 172812 | pLBT0160 | Level 1 module containing P19_P1 F |
| 172813 | pLBT0170 | Level 1 module containing nCFP_P4 F |
| 172814 | pLBT0180 | Level 1 module containing TP-CFP_P4 F |
| 172815 | pLBT0190 | Level 1 module containing OEP7-mTRQ_P4 F |
| 172816 | pLBM0010 | Level 2 module containing HSP21-GFP |
| 172817 | pLBM0020 | Level 2 module containing HSP21-nYFP HSP21-cYFP |
| 172818 | pLBM0030 | Level 2 module containing HSP21-nYFP PTAC5-cYFP |
| 172819 | pLBM0040 | Level 2 module containing HSP21-nYFP deltaPTAC5-cYFP |
| 172820 | pLBM0050 | Level 2 module containing mCHERRY-nYFP HSP21-cYFP |
| 172821 | pLBM0060 | Level 2 module containing HSP21-nYFP HSP21-cYFP OEP7-mTRQ |
| 172822 | pLBM0070 | Level 2 module containing mCHERRY-nYFP HSP21-cYFP OEP7-mTRQ |
| 172824 | pLBM0090 | Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS |