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A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo.

Wu Z, He K, Chen Y, Li H, Pan S, Li B, Liu T, Xi F, Deng F, Wang H, Du J, Jing M, Li Y
Neuron. 2021 Dec 17. pii: S0896-6273(21)00988-0. doi: 10.1016/j.neuron.2021.11.027. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167577pAAV-hsyn-GRAB_ATP1.0Express the ATP sensor GRAB_ATP1.0 in neurons
167578pAAV-hsyn-GRAB_ATP1.0mutExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons
167579pAAV-GfaABC1D-GRAB_ATP1.0Express the ATP sensor GRAB_ATP1.0 in astrocytes
167580pAAV-GfaABC1D-GRAB_ATP1.0mutExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes
167581pAAV-EFS-DIO-GRAB_ATP1.0Express the ATP sensor GRAB_ATP1.0 in Cre positive cells
167582pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAXExpress the ATP sensor GRAB_ATP1.0 in mammalian cells
167583pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAXExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

Antibodies from Article