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A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo.

Dong A, He K, Dudok B, Farrell JS, Guan W, Liput DJ, Puhl HL, Cai R, Wang H, Duan J, Albarran E, Ding J, Lovinger DM, Li B, Soltesz I, Li Y
Nat Biotechnol. 2021 Nov 11. pii: 10.1038/s41587-021-01074-4. doi: 10.1038/s41587-021-01074-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164604pAAV-hSyn-GRAB_eCB2.0Express the endocannabinoid sensor GRAB_eCB2.0 in neurons
164605pAAV-hSyn-GRAB_eCBmutExpress the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons
164606pAAV-hSyn-DIO-GRAB_eCB2.0Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons
164607pAAV-hSyn-DIO-GRAB_eCB2.0mutExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons
164608pAAV-CaMKII-GRAB_eCB2.0Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons
164609pAAV-GfaABC1D-GRAB_eCB2.0Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes
164610pAAV-EFS-fDIO-GRAB_eCB2.0Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells
164611pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAXExpress the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells
164612pDisplay-GRAB_eCBmut-IRES-mCherry-CAAXExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

Antibodies from Article